. MathSciNet The peritoneal micromilieu commits B cells to home to body cavities and the small intestine. Patients should be anesthetized. WO2023056606A1 - Valproate (vpa) compounds for use in treating In this study, intraperitoneal (IP) injection was used to mimic the human administration route. Histopathological alterations in the mouse brain were detected after . The accessibility of the therapeutic cells to their expectable target site is strongly dependent on the route of administration used. In our study, we found inflamed colon could induce more MSCs engraftment than heathy colon, which partly supported this hypothesis, since inflamed colon has been reported of releasing variety of chemotactic cytokines26. The most efficient method to inject small animals is a two-person method where one holds the rodent and the other person injects the rodent at about 10 to 20 degrees in mice and 20 to 45 degrees in rats. The results set a precedent of how other drugs may be delivered in this way to treat other similar medical issues after further research. After antigen retrieval, the endogenous peroxidase activity was blocked by 1% H2O2 for 10min. Wang Min, Liang Cong and Hu Hao contributed equally to this work. Interaperitoneal Administration of lpha-Tocopherol Loaded Nanoparticles Improves Ischemia-Reperfusion Injury in Rat Ovaries Torsion and Detorsion Model. Horizontal bars represent medians, boxes represent the 25th and 75th percentiles. Sirius red staining showed disorganized collagen fibers in DSS control colon, which were diffusely distributed in the injured colon wall. The IP injection showed longer colon, with mean length of 8.24cm compared with 6.90cm in AI group (P=0.0057) and 6.14cm in IV group (P<0.001; Fig. Intraperitoneal Drug Administration - an overview | ScienceDirect Topics Intraperitoneal injection. Histological evaluation was completed in a double-blind fashion by two independent pathologist. Article Skin application of non-rodents (miniature pigs) Collagen deposition is another indicator for wound healing, however, extensive collagen deposition may hamper the gut reconstitution14. Insular cortex is a critical brain region that participates in the interoceptive sensations. RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Bio Rad, Hercules, CA). HHS Vulnerability Disclosure, Help ", "Intraperitoneal injection (IP), Intravenous injection (IV) or anal injection (AI)? Though genetic and environmental factors are believed to be involved in the disease, not much progress has been made on therapy. Total RNA was extracted with the RNAeasy Plus kit (QIAGEN). Nature protocols 5, 550560, doi: 10.1038/nprot.2009.238 (2010). A disadvantage of using intraperitoneal injections is that they can have a large variability in effectiveness and misinjection. The histological score was defined as the sum of the two parameters (total score=E+I). Some research showed exogenous CD34 stem cells migration towards the inflamed colon and differentiation into endothelial cells40. Unable to load your collection due to an error, Unable to load your delegates due to an error. Before You are using a browser version with limited support for CSS. However, human insulin . For some severe cases, IBD may lead to the life-threaten complications5. Thus, IP and AI routes are free of pulmonary embolism issue. NIR fluorescent dye, 1, 1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide (DiR; DiIC18(7), Molecular Probes, Invitrogen, Carlsbad, CA) was used for labeling the cells as previously reported65. By using this techniques, the catheter complications, in particular obstruction, bowel and vaginal cuff perforation were greatly reduced61. However, no significant TSG-6 difference was found between AI and IV groups. Article East-West gradient in the incidence of inflammatory bowel disease in Europe: the ECCO-EpiCom inception cohort. How to cite this article: Wang, M. et al. A study was conducted to determine the best route of administration to transplant mesenchymal stem cells for colitis. We selected the day 3 as observation time point. GFP+ cells were detected at the inflamed colon. Disease recovery was evaluated by histological server score, gross body weight and survival rate. Although controversial, intraperitoneal use in ovarian cancer has been recommended as a standard of care. It can significant reduce failure puncture and associated organ damages caused by blind needle puncturing. Intraperitoneal foreign bodies such as suture material, clips, and mesh also contribute to adhesion formation. Secondly, as mentioned above, there are plenty of immune cells in peritoneal cavity28, which are indispensable component of MSCs aggregates. Journal of Crohns & colitis 6 Suppl 2, S224S234, doi: 10.1016/s1873-9946(12)60502-9 (2012). This review discusses the usefulness of IP drug therapy and the factors influencing it, as well as strategies to increase the efficacy, and conclude that IP route is an alternate route to the more conventional drug delivery routes, and can be successfully used when the target is within the peritoneal cavity or adjacent tissue. The IV group showed 20.3% ki-67+ cells/crypt. Furthermore, the corresponding mesentery lymph nodes (MLNs) also showed relatively high MSCs existence (Fig. Pentobarbital sodium Injection Caution. Stem Cell Res Ther 3, 51, doi: 10.1186/scrt142 (2012). Injections are given along the midline caudal to the umbilicus or low on the right lateral abdomen. [5] In 1967, the production of amnesia was studied through an injection of physostigmine. Unlike MSCs, they are restricted by migration because of receptors in the peritoneal cavity specially recognizing the PCs29,30. Mice are usu Role for interferon-gamma in the immunomodulatory activity of human bone marrow mesenchymal stem cells. Article Cardiovascular research 96, 208209; discussion 210203, doi: 10.1093/cvr/cvs202 (2012). Cancers | Free Full-Text | Targeted Vaccination against Human These complications include catheter infection, physical damages to intra-abdominal structures, such as vaginal vault perforation, bladder erosion and bowel perforation58 and direct drug toxicity to non-specific abdomen organ (e.g. Fischer, U. M. et al. In this study, intraperitoneal (IP) injection was used to mimic the human administration route. Cite this article. A., Ong, J. F. & Karp, J. M. Mesenchymal stem cells: immune evasive, not immune privileged. The good cell-cell cross talk between MSCs and immune cells may be another contributor for better therapeutic effects. Here, we combined the iDISCO + method and Fos immunostaining to confirm that the middle part of the right-side, but not the left-side, insular cortex in adult male mice is activated by intraperitoneal injection of lithium chloride. 2020 Apr;17(4):511-522. doi: 10.1080/17425247.2020.1736551. Streptozotocin-induced Diabetic Models In Mice And Rats Small animals: 1 mL per 10 lb of body weight. Afterwards, the samples were incubated with Ki-67 (1:200; Santa Cruz) or FOXP3 (1:100; Antibodies-Online, Aachen, Germany) overnight at 4C. Journal of biomedical optics 11, 050507, doi: 10.1117/1.2364903 (2006). This concern arises because MSCs has an estimated diameter of 2030m15,16. [8], A good example of how intraperitoneal injections work is depicted through "The distribution of salicylate in mouse tissues after intraperitoneal injection" because it includes information on how a drug can travel to the blood, liver, brain, kidney, heart, spleen, diaphragm, and skeletal muscle once it has been injected intraperitoneally.[9]. CAS 6B). study on peritoneal cells (PCs) migration to gut may give us some hints27. von Bahr, L. et al. Severity of DSS-induced colitis was also assessed using a score system by two independent pathologists. Gastroenterology 140, 17851794, doi: 10.1053/j.gastro.2011.01.055 (2011). If there is negative pressure, proceed with the injection depress the plunger until the solution has been fully administered. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. The following approaches are novel, and require further testing in humans before being accepted as potential treatment options: This study was designed (1) to examine a novel route of opioid administration, the intraperitoneal injection; (2) to compare this to interpleural application, and (3) to compare opioid with local anesthetic effects under both conditions.Methods. Intraperitoneal injection is widely used in rodents as a route of drug administration. Gastroenterology 114, 433440 (1998). Drug Deliv. [10] The results depicted that ketobemidone consumption was significantly lower when patients controlled anesthetic through IP. The Journal of infectious diseases 198, 456464, doi: 10.1086/590112 (2008). completed the main experiment and wrote the first draft of the paper. This shows an important limitation to the use of IP therapy. Further control study comparing bowel signals on healthy mice and DSS mice after MSCs injection showed weaker NIR signal on healthy bowel in all three routes, which indicated fewer MSCs migration to the colon without colitis (Supplementary Figure S5). This site needs JavaScript to work properly. An example of how intraperitoneal injections can be optimized is depicted in a study where IP injections are used to deliver anesthesia to mice. We even tried 1108 cell/mouse in IP and AI group and no immediate death was occurred. Nature medicine 18, 618623, doi: 10.1038/nm.2695 (2012). Ng, S. C. et al. Endoscopic fluorescence imaging experiments were performed at 1-h postinjection of FA-NPs, celecoxib/FA-NPs or FNC-NPs using a Karl Storz-Endoskope System. Taede et al. Intraperitoneal injection (IP), Intravenous injection (IV) or anal Subcutaneous Compared with Intraperitoneal Ketamine-Xylazine for Myd88-dependent positioning of Ptgs2-expressing stromal cells maintains colonic epithelial proliferation during injury. CAS doi: 10.1002/14651858.CD005284.pub3. In contrast, GFP+ cells were showed in the lumen for AI group and lamina propria for IV group (Fig. PDF Chris Quigley Essentials Full Spectrum Curriculum (2023) However, is IV injection the best way for MSCs delivery in IBD? Nature medicine 5, 309313, doi: 10.1038/6529 (1999). Please enable it to take advantage of the complete set of features! Interferon-gamma (IFN-gamma)- and tumour necrosis factor (TNF)-induced nitric oxide as toxic effector molecule in chronic dextran sulphate sodium (DSS)-induced colitis in mice. & Prockop, D. J. Anti-inflammatory protein TSG-6 secreted by activated MSCs attenuates zymosan-induced mouse peritonitis by decreasing TLR2/NF-kappaB signaling in resident macrophages. evaluated the therapeutic efficacy of different MSCs sources and delivery routes in experimental emphysema37. The Ki-67+ cells were greatly increased after MSCs treatment. Intraperitoneal Injection of Oncolytic Viruses H101 for Patients With Best way for mesenchymal stem cells transplantation for colitis", "Intraperitoneal injections as an alternative method for micro-CT contrast enhanced detection of murine liver tumors", "Optimization of Intraperitoneal Injection Anesthesia in Mice: Drugs, Dosages, Adverse Effects, and Anesthesia Depth", "Intraperitoneal Injection Is Not Always a Suitable Alternative to Intravenous Injection for Radiotherapy", "Pharmacological principles of intraperitoneal and bidirectional chemotherapy", Transjugular intrahepatic portosystemic shunt, https://en.wikipedia.org/w/index.php?title=Intraperitoneal_injection&oldid=1142986299, Short description is different from Wikidata, Articles with unsourced statements from April 2015, Articles with unsourced statements from January 2022, Creative Commons Attribution-ShareAlike License 3.0, This page was last edited on 5 March 2023, at 09:43.

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